Research Methods

Methods & Techniques
Our tools enable us to utilise the following methods:
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Surgical examination of rodents, cannulation, cerebrospinal fluid and blood collection
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Stereotoxic operations, brain lesions, pathway transection
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Chemogenetics, viral injections
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Behavioral tests of emotional changes and maternal motivation of animals
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Automatic video analysis
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Continuous monitoring of behavior with Intellicage
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Neuronal pathway tracing using transgenic mice and viral techniques
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Laser microdissection, RT-PCR, real-time fluorescent PCR
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Bioinformatics of systems biological data
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RNA amplification for DNA microarray and sequencing
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In vitro electrophysiology, patch-clamp technique
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Molecular Biology Techniques
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In situ hybridization histochemistry, autoradiography
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Immunostaining and fluorescence microscopy
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Western blot techniques
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in vivo fiberphotometry
Demonstration of the combination of confocal and electron microscopy techniques
Parathyroid hormone 2 neuropeptide-containing fibres synapse with oxytocin neurons in the paraventricular hypothalamic nucleus (PVN).