Research Methods

Methods & Techniques

Our tools enable us to utilise the following methods:

  • Surgical examination of rodents, cannulation, cerebrospinal fluid and blood collection

  • Stereotoxic operations, brain lesions, pathway transection

  • Chemogenetics, viral injections

  • Behavioral tests of emotional changes and maternal motivation of animals

  • Automatic video analysis

  • Continuous monitoring of behavior with Intellicage

  • Neuronal pathway tracing using transgenic mice and viral techniques

  • Laser microdissection, RT-PCR, real-time fluorescent PCR

  • Bioinformatics of systems biological data

  • RNA amplification for DNA microarray and sequencing

  • In vitro electrophysiology, patch-clamp technique

  • Molecular Biology Techniques

  • In situ hybridization histochemistry, autoradiography

  • Immunostaining and fluorescence microscopy

  • Western blot techniques

Demonstration of the combination of confocal and electron microscopy techniques

Parathyroid hormone 2 neuropeptide-containing fibres synapse with oxytocin neurons in the paraventricular hypothalamic nucleus (PVN).